Rough-toothed dolphins (Steno bredanensis) are reported to form isolated communities with stable groups around some oceanic islands. Within these communities, this species exhibits cooperative foraging and care-giving behavior including the sharing of large prey. These group behaviors could be driving community level structure where there are clear differences in habitat use between island groups, and limited movement among islands. Similar social characteristics are found in other cetacean species, e.g. killer whales, where individuals remain in maternally related groups indefinitely, forming “extended matrilines” as reflected by a single mitochondrial (mt) DNA haplotype. Here we test the hypothesis that rough-toothed dolphins also form “extended matrilines” using mtDNA control region haplotypes as a marker for maternal lineages. Alternatively, we test the influence of maternal fidelity on group structure within communities. The sample collection comprises adults from Pacific Ocean Islands and the continental Atlantic USA spanning 1976-2010. We sequenced mtDNA from 4 deceased groups (3 mass strandings and 1 bycatch; 350bp; n=28 teeth samples) and 14 living groups (460bp; n=106 skin samples) identifying 22 haplotypes. Differentiation and haplotype diversity were evaluated for groups within island communities in the Hawaiian Islands (Kauai and Hawaii) and Society Islands (Moorea and Raiatea) as well as between islands communities. Our results revealed multiple matrilines in 16 of the 18 groups, allowing us to reject the hypothesis of strict matrilineal structure. Although an AMOVA detected significant differentiation between island communities, groups did not significantly partition any genetic diversity within island communities. This is consistent with permutation tests where no strong signal of matrilineal influence was identified within island communities. Our study provides evidence of genetic structure at the community level, but demonstrates negligible structure at the group level. Fine-scale analyses to evaluate kinship and temporal association patterns are underway to further our understanding of group structure for this species.

This report describes results from vessel surveys and visual species-verification trials conducted at the Jacksonville Shallow Water Training Range (JSWTR) in the Jacksonville Operating Area (OPAREA). This work continues from a multi-institutional monitoring program intended to provide information on the species composition, population identity, density, and baseline behavior of marine mammals and sea turtles present in United States (U.S.) Navy range complexes along the U.S. Atlantic Coast.

Primary objectives of this project have included the following: Collect baseline occurrence data (location, sex, group size, behavior) of humpback whales (and other species of baleen whales, opportunistically), Obtain identification photographs of humpback whales for inclusion in regional and local catalogs, Collect biopsy samples of humpback whales for sex determination, mitochondrial control region sequencing and microsatellite genotyping of tissue samples, and stable isotope analysis to assess foraging related to prey consumption • Conduct satellite tagging to document seasonal humpback whale movement patterns in the nearshore waters off Virginia Beach, specifically whether the whales spend significant time in areas of high shipping traffic and/or areas of U.S. Navy training exercises

A total of 23 nearshore and mid-shelf vessel surveys were completed during the 2023/24 field season. In total, 43 baleen whale sightings, including 33 sightings of humpback whales (Megaptera novaeangliae) composed of 43 individuals, 6 sightings of North Atlantic right whales (Eubalaena glacialis) composed of 9 individuals (1 deceased), 3 sightings of minke whales (Balaenoptera acutorostrata) composed of 3 individuals, and 1 sighting of a fin whale (Balaenoptera physalus) composed of 1 individual occurred during the 2023/24 field season.

The objective of this multi-year project under the U.S. Navy’s Marine Species Monitoring Program has been to establish baseline information on occurrence and behavior of humpback whales near Naval Station Norfolk and within the Virginia Capes (VACAPES) Operating Area

The objective of this multi-year project under the U.S. Navy’s Marine Species Monitoring Program has been to establish baseline information on occurrence and behavior of humpback whales near Naval Station Norfolk and within the Virginia Capes (VACAPES) Operating Area (OPAREA)

The objective of this multi-year project under the U.S. Navy’s Marine Species Monitoring Program has been to establish baseline information on occurrence and behavior of humpback whales near Naval Station Norfolk and within the Virginia Capes (VACAPES) Operating Area by addressing the following questions: What age classes (juveniles, sub-adults, adults) are utilizing the waters within and adjacent to the mouth of the Chesapeake Bay? Do humpback whales exhibit site fidelity over periods of days to years? Do humpback whales congregate in specific high-traffic and/or high-use U.S. Navy training areas? Do humpback whales spend significant time within or move through areas of U.S. Navy live-fire and mine neutralization exercise (MINEX) training?

The objective of this multi-year project under the U.S. Navy’s Marine Species Monitoring Program has been to establish baseline information on occurrence and behavior of humpback whales near Naval Station Norfolk and within the Virginia Capes (VACAPES) Operating Area by addressing the following questions: What age classes (juveniles, sub-adults, adults) are utilizing the waters within and adjacent to the mouth of the Chesapeake Bay? Do humpback whales exhibit site fidelity over periods of days to years? Do humpback whales congregate in specific high-traffic and/or high-use U.S. Navy training areas? Do humpback whales spend significant time within or move through areas of U.S. Navy live-fire and mine neutralization exercise (MINEX) training?

A total of 22 nearshore and mid-shelf vessel surveys were completed during the 2024/25 field season. In total, 60 baleen whale sightings, including 40 sightings of humpback whales (Megaptera novaeangliae) totaling 59 individuals, 10 sightings of North Atlantic right whales (NARWs; Eubalaena glacialis) totaling 22 individuals, 7 sightings of fin whales (Balaenoptera physalus) totaling 14 individuals, and 3 sightings of individual minke whales (Balaenoptera acutorostrata), occurred during the 2024/25 field season. A total of 24 aerial surveys were flown during the 2024/25 field season in association with this effort and the Offshore Cetacean Study (OCS) (A. Engelhaupt et al. 2026); see Ozog and Engelhaupt 2026 for additional details regarding the aerial surveys. Baleen whale sightings from the aerial surveys included humpback (n=45), NARWs (n=8), fin (n=11), and minke (n=3) whales (Ozog and Engelhaupt 2026). During the OCS surveys, baleen whale sightings included fin whales (n=12), humpback whales (n=7), a blue whale (Balaenoptera musculus; n=1), a minke whale (n=1), and an NARW (n=1) (A. Engelhaupt et al. 2026). Across this project and the OCS effort, satellite-linked telemetry tags were deployed on one humpback whale, one fin whale, and one NARW. Customized Animal Tracking Solution tags were deployed on two NARWs and two humpback whales. One biopsy sample was collected from the satellite-tagged humpback whale.

Since January 2015, HDR Inc. has been monitoring humpback whales (Megaptera novaeangliae) to assess their occurrence, habitat use, and behavior within and near United States (U.S.) Navy training and testing areas off Virginia via the Mid-Atlantic Humpback Whale Monitoring Project.

Since January 2015, HDR Inc. has been monitoring humpback whales (Megaptera novaeangliae) to assess their occurrence, habitat use, and behavior within and near United States (U.S.) Navy training and testing areas off Virginia via the Mid-Atlantic Humpback Whale Monitoring Project.

As part of the long-term United States (U.S.) Navy-funded Marine Species Monitoring Program, from 11–19 February 2024, Cascadia Research Collective (CRC) carried out a vessel-based field effort in conjunction with passive acoustic monitoring undertaken by the U.S. Navy (Navy) scientists on and around the underwater hydrophone ranges of the Pacific Missile Range Facility (PMRF). The effort was timed to occur immediately prior to the start of a Submarine Command Course (SCC) to allow for collection of movement and dive data that could be used to examine exposure and response of cetaceans to Navy mid-frequency active sonar (MFAS; see Henderson et al. 2021). Eight days of field effort were funded by the Navy, and one day of field work was funded by National Oceanic and Atmospheric Administration (NOAA) Fisheries. Wildlife Computers SPLASH10-F satellite tags were funded by the Navy for deployment on Navypriority species, and additional tags were provided by Wildlife Computers or CRC for deployment on other species (e.g., humpback whales [Megaptera novaeangliae]). Tags were programmed to collect dive behavior and Fastloc®-Global Positioning System (GPS) data from the time deployed until three and a half days after the end of the SCC and continue to transmit the dive and Fastloc®-GPS data for an additional six days. Starting on either May 2nd or May 3rd, the tags were duty cycled to transmit only every second day, to provide longer-term tracks of individual movements. During the period that tags were collecting dive and Fastloc®-GPS data, tags were programmed to try to obtain Fastloc®-GPS locations only for the 12 hours of the day when there were no or limited satellite overpasses. This programming change from previous Kauaʻi field efforts was taken to minimize temporal gaps in location data during the period that overlapped with and immediately followed the SCC. Field efforts were undertaken out of Kikiaola small boat harbor when conditions allowed for working on or near PMRF, or out of Port Allen when conditions on or near PMRF were not workable.

Movements and Spatial Use of Satellite‐tagged Odontocetes in the Western Main Hawaiian Islands: Results of Fieldwork Undertaken off O‘ahu in October 2010 and Kaua‘i in February 2011

Although considerable information is available on residency patterns and spatial use of odontocetes in the eastern half of the Hawai‘i Range Complex (HRC), much less is known about odontocetes in the western half of the HRC. In the second year of a three-year effort in the western main Hawaiian Islands we undertook surveys off Kaua‘i and Ni‘ihau in July/August 2011, to examine spatial use and residency patterns using satellite tags, to provide visual verification of acoustically-detected odontocetes on the Pacific Missile Range Facility (PMRF), and to obtain individual identification photographs and biopsy samples for assessment of population identity and structure.

Here we combine results of field efforts funded through the U.S. Navy’s Living Marine Resources Program (through a three-year grant from the Naval Postgraduate School) as well as additional field time in the study area in 2011 and 2012 funded through the Pacific Islands Fisheries Science Center and Commander, Pacific Fleet. We also utilize results from research efforts in the eastern main Hawaiian Islands, off Lāna‘i, Maui, and Hawai‘i Island, to help interpret results from photo-identification and satellite tagging.

A joint project in July and August 2013 on and around the Pacific Missile Range Facility (PMRF) combined passive acoustic monitoring and boat-based field efforts. There were 671 kilometers (36.6 hours [hr]) of small-vessel survey effort over the course of the 8-day project, with 55.1 percent of time (20.2 hr) spent within the PMRF instrumented hydrophone range boundaries. A total of 33.0 hr of acoustic monitoring coincided with the small-vessel field effort.

A joint project in February 2014 on and around the Pacific Missile Range Facility (PMRF) was carried out utilizing combined passive acoustic monitoring and boat-based field efforts. There were 1,287 kilometers (km) (66.3 hours [hr]) of small-vessel survey effort over the course of the 10 day project, with 44.6 percent of search time (29.6 hr) spent within the PMRF instrumented hydrophone range boundaries. A total of 81.7 hr of acoustic monitoring was undertaken during the field effort. There were 26 sightings of five species of odontocetes, six of which were directed by acoustic detections from the Marine Mammal Monitoring on Navy Ranges (M3R) system.

As part of a long-term U.S. Navy-funded marine mammal monitoring program, in February 2016 a combining boat-based field effort and passive acoustic monitoring was carried out on and around the Pacific Missile Range Facility (PMRF). The U.S. Navy funded five days of small-boat effort and the National Marine Fisheries Service funded an additional two days of effort. There were 859 kilometers (49 hours) of small-vessel survey effort over the course of the seven day project.

As part of a long-term U.S. Navy-funded marine mammal monitoring program, in August 2017 a combination of boatbased field effort and passive acoustic monitoring was carried out on and around the Pacific Missile Range Facility (PMRF) off Kaua‘i prior to a Submarine Command Course scheduled for mid-August 2017. The U.S. Navy funded five days of small-boat effort and the National Marine Fisheries Service funded an additional six days of effort. There were 1,113 kilometers (77.4 hours) of small-vessel survey effort over the course of the 11 day project.

As part of a long-term U.S. Navy-funded marine mammal monitoring program, in August 2018 a combination of vessel-based field effort and passive acoustic monitoring was carried out on and around the Pacific Missile Range Facility (PMRF) Kaua‘i prior to a Submarine Commander Course scheduled for mid-August 2018. The purpose of the monitoring effort was to assess the spatial movement patterns and habitat use of cetaceans that are exposed to mid-frequency active sonar and how those patterns influence exposure and potentially responses.

As part of a long-term U.S. Navy-funded marine mammal monitoring program, in February 2020 a combination of vesselbased field effort and passive acoustic monitoring was carried out on and around the Pacific Missile Range Facility (PMRF) off Kaua‘i prior to a Submarine Command Course scheduled for mid-February 2020. The purpose of the monitoring effort was to assess the spatial movement patterns and habitat use of cetaceans that are exposed to midfrequency active sonar and how those patterns influence exposure and potentially responses. Results from this effort were compared with previous Cascadia Research Collective (CRC) survey effort and photo-identification and tag data from Kaua‘i, based on surveys in 11 different years since 2003.

The goals of this study were to conduct photographic-identification (photo-ID) surveys during 2015 and 2016 to determine abundance, distribution patterns, habitat use, and site fidelity of bottlenose dolphins in St. Andrew Bay and adjacent coastal waters in the NSWC PCD Testing Range over four sampling periods (July and October 2015, and April and October 2016).

This report summarizes small boat based research conducted on cetaceans off southern California by the Scripps Institution of Oceanography (SIO) in collaboration with Southwest Fisheries Science Center (SWFSC) from August 2009 – July 2010. The primary objectives of this research were to use sighting, photo-identification, biopsy and acoustical sampling techniques to assess the occurrence, distribution and population structure of small cetaceans in a region that is subject to frequent naval exercises; this information is needed to evaluate possible effects from Mid Frequency Active Sonar (MFAS) trials and ultimately for the development of appropriate management protocols.

The first cetacean circovirus, beaked whale circovirus (BWCV), was recently reported in a Longman’s beaked whale (Indopacetus pacificus) stranded in Hawai‘i and represents an emergent disease with unknown population impacts. In other species, circovirus infection may cause mortality or opportunistic co-infection by other pathogens. We report on a targeted surveillance of stranded cetaceans in the Pacific basin, including archived beaked whale species, strandings where pathological findings suggested disease presence, mass stranded animals, and additional individuals to represent a broad range of Hawaiian cetacean species. Archived tissues primarily from the brain, kidney, liver, lung, spleen, and lymph nodes of individuals stranded between 2000 and 2020 (n=30) were tested by PCR for the presence of BWCV.

Remote biopsy sampling is a common method used to obtain tissue samples from wild cetaceans. Using this technique, researchers typically obtain a small sample of skin and blubber using a biopsy tip fired from a crossbow or modified air rifle. Analysis of these tissues can provide important information on specific identity, sex, pollutant levels, diet, and reproductive status, which are critical to studies of free-ranging cetaceans. Biopsy sampling is generally considered to be a relatively benign procedure, but all prior attempts to evaluate its impact have been subjective assessments of the behavioral response of individuals at the surface. The goal of the present study is to provide a quantitative assessment of the immediate effects of biopsy attempts on the behavior of short-finned pilot whales (Globicephala macrorhynchus) equipped with digital acoustic recording tags (DTags) off Cape Hatteras, North Carolina. A biopsy attempt was defined as any instance of contact between a biopsy dart with an animal. A series of five metrics was examined to determine if behavior of whales was affected by a biopsy attempt, including: foraging behavior (number of dives, depth, and number of prey capture attempts); time spent within 3 m of the surface; fine-scale body orientation; fluke rate and amplitude; and group vocalization rate. The short-term reactions to biopsy attempts appear to be ephemeral and should not compromise the fitness of the animal, although the effects of increasing the group vocalization rate after a biopsy attempt should be examined further. The results of this analysis provide the first subsurface, quantitative assessment of the short-term effects of biopsy sampling on cetaceans.

Remote biopsy sampling has been used for almost 40 yr (Winn et al. 1973) to obtain tissue samples for various analyses, and is particularly important because most cetaceans are difficult or impossible to capture safely at sea (Noren and Mocklin 2011). It is generally assumed that there are few, if any, detrimental effects on the fitness of sampled animals. In support of this assumption, there has only been one documented case of death of a cetacean from a biopsy dart (Bearzi 2000), although it is possible deaths have occurred that have not been described in the published literature. Failure of the dart stopper and the poor body condition of the common dolphin (Delphinus delphis) were believed responsible for the death of this animal, as the dart penetrated through its thin blubber layer.

In the Pacific, the Marine Mammal Monitoring on Navy Ranges (M3R) program maintains systems that continuously run algorithms to detect, classify and localize marine mammals year-round on the U.S. Navy’s deep-water Southern California Antisubmarine Warfare Range (SOAR) in Southern California and the Pacific Missile Range Facility (PMRF) off Kaua‘i, Hawai‘i. Long-term archive data collected on these ranges allow for numerous types of studies on species inhabiting the ranges, including the monitoring of abundance, behavioral responses to naval activities, and habitat usage. They also provide the opportunity to study ambient sound levels and soundscapes. In FY24 the M3R program had five goals for SOAR and PMRF: Support real-time monitoring of on-water tagging operations Collection and postprocessing of M3R binary archives and periodic broadband recordings Data preservation: conversion of Alesis recordings to M3R packet recorder format SOAR Continuous Active Sonar Behavioral Response Study (CAS BRS) DICASS sonobuoy receiver adaption in preparation for deployment at SOAR.

In the Pacific, the Marine Mammal Monitoring on Navy Ranges (M3R) program maintains systems that continuously run algorithms to detect, classify and localize marine mammals year-round on the U.S. Navy’s deep-water Southern California Antisubmarine Warfare Range (SOAR) in Southern California and the Pacific Missile Range Facility (PMRF) off Kaua‘i, Hawai‘i. Long-term archive data collected on these ranges allow for numerous types of studies on species inhabiting the ranges, including the monitoring of abundance, behavioral responses to naval activities, and habitat usage. They also provide the opportunity to study ambient sound levels and soundscapes.

Taking into consideration the multiple intermediate scientific objectives in the U.S. Navy’s Strategic Planning Process (DoN 2013), the goals of this study are to assist the U.S. Navy and regulatory agencies with environmental planning and compliance by addressing the following questions: Which cetacean species occur within the VACAPES OPAREA off Virginia, and how does occurrence fluctuate seasonally? What are the baseline behaviors and ecological relationships of offshore cetaceans within the study area? Do individual cetaceans exhibit site fidelity within specific regions of the study area over periods of weeks, months, or years? What is the seasonal extent of cetacean movements within and around U.S. Navy VACAPES training range boxes? Do cetaceans spend significant time within or primarily move through areas of U.S. Navy live-fire or Anti-Submarine Warfare training events?

Taking into consideration the multiple intermediate scientific objectives in the U.S. Navy’s Strategic Planning Process (DoN 2013b), the goals of this study are to assist the U.S. Navy and regulatory agencies with environmental planning and compliance by addressing the following questions: • Which cetacean species occur over the outer continental shelf to the east of NSN, and how does occurrence fluctuate seasonally? • What are the baseline behaviors and ecological relationships of offshore cetaceans within the study area? • Do individual cetaceans exhibit site fidelity within specific regions of the study area over periods of weeks, months, or years? • What is the seasonal extent of cetacean movements within and around U.S. Navy VACAPES training range boxes? • Do cetaceans spend significant time within or primarily move through areas of U.S. Navy live-fire or Anti-Submarine Warfare training events?

Taking into consideration the multiple intermediate scientific objectives in the U.S. Navy’s Strategic Planning Process (DoN 2013b), the goals of this study are to assist the U.S. Navy and regulatory agencies with environmental planning and compliance by addressing the following questions:  Which cetacean species occur over the outer continental shelf to the east of NSN, and how does occurrence fluctuate seasonally?  What are the baseline behaviors and ecological relationships of offshore cetaceans within the study area?  Do individual cetaceans exhibit site fidelity within specific regions of the study area over periods of weeks, months, or years?  What is the seasonal extent of cetacean movements within and around U.S. Navy VACAPES training range boxes?  Do cetaceans spend significant time within or primarily

Taking into consideration the multiple intermediate scientific objectives in the U.S. Navy’s Strategic Planning Process (DoN 2013b), the goals of this study are to assist the U.S. Navy and regulatory agencies with environmental planning and compliance by addressing the following questions:  Which cetacean species occur over the outer continental shelf to the east of NSN, and how does occurrence fluctuate seasonally?  What are the baseline behaviors and ecological relationships of offshore cetaceans within the study area?  Do individual cetaceans exhibit site fidelity within specific regions of the study area over periods of weeks, months, or years?  What is the seasonal extent of cetacean movements within and around U.S. Navy VACAPES training range boxes?  Do cetaceans spend significant time within or primarily

Taking into consideration the multiple intermediate scientific objectives in the U.S. Navy’s Strategic Planning Process (DoN 2013b), the goals of this study are to assist the U.S. Navy and regulatory agencies with environmental planning and compliance by addressing the following questions:  Which cetacean species occur over the outer continental shelf to the east of NSN, and how does occurrence fluctuate seasonally?  What are the baseline behaviors and ecological relationships of offshore cetaceans within the study area?  Do individual cetaceans exhibit site fidelity within specific regions of the study area over periods of weeks, months, or years?  What is the seasonal extent of cetacean movements within and around U.S. Navy VACAPES training range boxes?  Do cetaceans spend significant time within or primarily

Taking into consideration the multiple intermediate scientific objectives in the U.S. Navy’s Strategic Planning Process (DoN 2013b), the goals of this study are to assist the U.S. Navy and regulatory agencies with environmental planning and compliance by addressing the following questions:  Which cetacean species occur over the outer continental shelf to the east of NSN, and how does occurrence fluctuate seasonally?  What are the baseline behaviors and ecological relationships of offshore cetaceans within the study area?  Do individual cetaceans exhibit site fidelity within specific regions of the study area over periods of weeks, months, or years?  What is the seasonal extent of cetacean movements within and around U.S. Navy VACAPES training range boxes?  Do cetaceans spend significant time within or primarily

Taking into consideration the multiple intermediate scientific objectives in the U.S. Navy’s Strategic Planning Process (DoN 2013), the goals of this study are to assist the U.S. Navy and regulatory agencies with environmental planning and compliance

Results from the second year of a three-year project investigating the distribution, demographics, and behavior of cetaceans in the US Navy’s Southern California operational area are summarized. Eighteen small vessel surveys for cetaceans, which included species verification tests in conjunction with M3R (Marine Mammal Monitoring on Navy Undersea Ranges) acoustic monitoring at the Southern California Anti-submarine Warfare Range (SOAR), as well as photo-identification, satellite tagging, and biopsy sampling of species of interest, were conducted in July 2011 and January and March 2012. 112 groups of 14 cetacean species were encountered. Twenty-one satellite tags, some depth reporting, were deployed on four species, with an emphasis on Cuvier’s beaked and fin whales. Among other findings, preliminary results of photo-identification studies combined with results from satellite tag data suggest that both Cuvier’s beaked whales and fin whales may have population sub-units with higher than expected residency to the Southern California Bight. Beaked whales particularly show this higher than expected residency to SOAR.

This report forms part of a multi-institutional monitoring project intended to provide information on the species composition, population identity, density and baseline behavior of marine mammals and sea turtles present in United States (U.S.) Navy range complexes along the U.S. 5 Atlantic coast.

This report describes results of vessel surveys from a multi-institutional monitoring project intended to provide information on the species composition, population identity, density, and baseline behavior of marine mammals and sea turtles present in United States (U.S.) Navy range complexes along the U.S. Atlantic Coast.

This report describes results of vessel surveys from a multi-institutional monitoring project intended to provide information on the species composition, population identity, density, and baseline behavior of marine mammals and sea turtles present in United States (U.S.) Navy range complexes along the U.S. Atlantic Coast.

This report describes results from a multi-institutional monitoring project intended to provide information on the species composition, population identity, density and baseline behavior of marine mammals and sea turtles present in United States (U.S.) Navy range complexes along the U.S. Atlantic Coast. This program began in 2007, with baseline aerial and vessel surveys and a passive acoustic monitoring program in Onslow Bay, North Carolina, and has since expanded to include study areas off Jacksonville, Florida and Cape Hatteras, North Carolina.

Understanding the spatio-temporal movements of animals is an integral component of wildlife conservation and management. Marine turtles are species of conservation concern and satellite telemetry is a primary research tool used to study their movements, providing high accuracy location data in near “real time”, thus facilitating rapid identification of movements and key habitats.

The first phase to integrate the historical sum of these U.S. Navy-sponsored visual survey efforts for marine species monitoring in the HRC was prepared by Uyeyama (2011). This report represents the second phase of that effort and presents an overview of monitoring survey effort to date in the HRC. Available data from visual surveys performed by both contractor-led efforts and U.S. Navy in-house surveys was compiled and processed in order to: 1. Create a geographic information system (GIS)-based summation of all survey tracks that can be easily visualized. 2. Construct a geo-referenced database of sightings that can be queried by species, observer platform, Beaufort Sea State (BSS), season, or other sighting/survey variables. 3. Conduct an enhanced analysis of sighting success with respect to survey platform, field protocol, and environmental conditions. 4. Inform and adjust data collection procedures on future surveys as needed to reflect lessons learned from sighting data compilation efforts presented here. 5. Provide information to enhance informed management of marine protected species in the HRC and support the adaptive management process that is part of the ICM

Beaked whales (Family Ziphiidae; Order Cetacea) are the least well-described group of marine mammals because they conduct very long deep dives, spend little time on the surface where they display cryptic behavior, and are difficult to identify due to the similarity between species. Beaked whales are also known to be susceptible to high-energy sound sources in the ocean (e.g. seismic research, sonars used by military activities).

This report summarizes the field effort from 15 – 28 November, 2020, to locate and document a species of beaked whales that had previously only been acoustically detected. While the species of interest was again recorded on acoustic drift buoys, a previously undescribed species of beaked whale was unexpectedly encountered instead. This new species has external morphological characteristics and a new echolocation pulse type that do not match any previously observed or recorded species and therefore may represent a newly described species. In addition to this species of beaked whale, we encountered and photographed three species of dolphin as well as humpback whales, and recorded two other species of beaked whales on the acoustic recorders.

An expedition was conducted with the goal of visually locating, acoustically recording, and obtaining biopsy samples of the Mesoplodon beaked whales that were encountered in 2020 and produce BWB pulses, as well as locating the beaked whales that produce the BW43 echolocation pulse in order to conclusively determine what species produce each of the pulse types.

The 2018 California Current Ecosystem Survey (CCES) was a joint project of the Marine Mammal and Turtle Division (MMTD) and the Fisheries Resources Division (FRD) of National Oceanographic and Atmospheric Administration’s (NOAA) Southwest Fisheries Science Center (SWFSC). The survey was conducted over the course of 7 legs aboard the NOAA ship Reuben Lasker between 26 June and 4 December 2018 (Table 1). CCES was an assessment survey for coastal pelagic fish stocks, marine mammals, seabirds, and oceanography along the west coasts of southern Canada (Vancouver Island), US, and northern Mexico (Baja California), out to a distance of approximately 200 nautical miles offshore (Fig. 1). MMTD and FRD worked jointly aboard the vessel during Legs 1 through 4 (OMAO Project No. RL-18-03) (Tables 1, 2a, and 2b), during which the vessel surveyed off the coasts of Vancouver Island and the US West Coast. Only MMTD operations were conducted during Legs 5 through 7 (OMAO Project No. RL-19- 01), during which the vessel surveyed off the US West Coast and Mexico. This document covers the work conducted by MMTD (MMTD Survey No. 1651). Work conducted by FRD is presented separately (Stierhoff et al. 2019).

The PIFSC CRP has conducted research on cetaceans in the Mariana Archipelago since 2010. A cooperative effort with funding from U.S. Navy Pacific Fleet and PIFSC has included summer and winter small-boat surveys off the southernmost islands (Saipan, Tinian, Aguijan, Rota, and Guam), development of photo-ID catalogs, analysis of collected tissue samples and satellite telemetry tag data, and shipboard visual and PAM surveys in portions of the EEZ in 2010 and 2015. PIFSC has maintained long-term PAM on moored recorders off Saipan and Tinian (since 2010) and off Pagan (since 2015) as part of the Pacific Islands Passive Acoustic Network (PIPAN). The goal of these efforts is to collect the data necessary to conduct population assessments for cetaceans within the Mariana Archipelago, including the determination of their occurrence, population structure and abundance, movements, distribution, and habitat use.

The Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) has been conducting visual surveys and long-term acoustic monitoring for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) as part of an ongoing effort to develop a record of cetacean occurrence in the region. Visual surveys have been conducted aboard small boats (7.6–12.2 m) since 2010 off the southernmost islands of the Mariana Archipelago (Guam, Rota, Saipan, Tinian, and Aguijan). These surveys include the collection of photographs for individual identification, tissue samples for genetic analysis of population structure, and the deployment of satellite tags for assessment of individual movements throughout the broader region.

The Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) has been conducting visual surveys and long-term acoustic monitoring for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) as part of an ongoing effort to develop a record of cetacean occurrence in the region. Visual surveys have been conducted aboard small boats (7.6-12.2 m) since 2010 off the southernmost islands of the Mariana Archipelago (Guam, Rota, Saipan, Tinian, and Aguijan). These surveys include the collection of photographs for individual identification, tissue samples for genetic analysis of population structure, and the deployment of satellite tags for assessment of individual movements throughout the broader region

The Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) has been conducting visual surveys and long-term acoustic monitoring for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) as part of an ongoing effort to develop a record of cetacean occurrence in the region. Visual surveys have been conducted aboard small boats (7.6-12.2 m) since 2010 off the southernmost islands of the Mariana Archipelago (Guam, Rota, Saipan, Tinian, and Aguijan). These surveys include the collection of photographs for individual identification, tissue samples for genetic analysis of population structure, and the deployment of satellite tags for assessment of individual movements throughout the broader region.

The Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) has been conducting visual surveys for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) and collecting long-term passive acoustic monitoring data at two sites in CNMI as part of an ongoing effort to develop a record of cetacean occurrence in the region. Visual surveys, conducted aboard small boats (7.6 – 12.2 m), have been ongoing since 2010 off the southern Mariana Islands of Guam, Rota, Saipan, Tinian, and Aguijan. T

an effort to further develop a record of cetacean occurrence in the region, as well as to gather photos and biopsy samples and deploy satellite tags for population studies, the Pacific Islands Fisheries Science Center's (PIFSC) Cetacean Research Program (CRP) conducted surveys for cetaceans in the waters surrounding Guam and the CNМІ (Figure 1) during 2010-2014. This research was carried out in partnership with the U.S. Navy and is expected to continue through 2015. Detailed reports for each survey year were submitted to the Navy (Oleson and Hill 2010, Ligon et al. 2011, Hill et al. 2012, Hill et al. 2013).

e Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) conducted surveys for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) in an effort to further develop a record of cetacean occurrence in the region.1 This was the fourth year conducting cetacean surveys aboard small boats (7.6 – 12.2 m) around the southern Mariana Islands of Guam, Rota, Saipan, Tinian, and Aguijan (Fig. 1). This project was carried out in partnership with the Commander, U.S. Pacific Fleet by a team of four primary personnel (Table 1). The collected data are being used to reveal the occurrence and distribution, stock structure, and movements of cetaceans within the study area.

The Pacific Islands Fisheries Science Center’s (PIFSC) Cetacean Research Program (CRP) conducted surveys for cetaceans in the waters surrounding Guam and the Commonwealth of the Northern Mariana Islands (CNMI) (Figure 1) in an effort to further develop a record of cetacean occurrence in the region and to gather photos and biopsy samples for population studies. This project was carried out in partnership with the U.S. Navy Commander, Pacific Fleet by a team of six primary personnel (Table 1).

Goals: Understand species occurrence and distribution. Determine the population structure and abundance of the those species. Study the movements and habitat use of individuals and populations. Methods: Small boat-based and ship-based surveys. Passive acoustic recordings (HARPs & towed array). Photo-ID, biopsy sampling, and satellite tagging.

The U.S. Navy represented by the Pacific Fleet Environmental Directorate, Chief of Naval Operations Environmental Readiness and Energy, Office of Naval Research, and Naval Postgraduate School, fund significant marine mammal monitoring programs within California. The bulk of this effort occurs within the Southern California Bight. In an endeavor coordinated with the U.S. National Marine Fisheries Service (NMFS) to address specific questions, efforts are underway to deploy a variety of research tools as a collection of focused, collaborative studies. Monitoring methods include research elements designed to collect data in support of region-specific monitoring and also contribute to the overall body of marine mammal knowledge. Research elements include visual surveys from airplanes and vessels, passive acoustic monitoring from bottom-mounted systems, photolD, biopsies, and marine mammal tagging. The framework for monitoring and results from August 2008 to August 2010 will be presented. During this period over 35,570 nautical miles of ocean were visually surveyed representing 2,285 hours of effort. There were 2,714 sightings of an estimated 155,375 marine mammals. Over 29,700 hours of passive acoustic recordings were obtained and are undergoing continued analysis. In addition, 24,000 digital photographs were taken of marine mammals, and 18 hours of digital video taken of various marine mammal behaviors. There were also 139 individual tissue biopsies, and 31 satellite tracking tags attached to various species. Continuing efforts in 2011 will employ many of the same techniques used previously. The Navy's annual monitoring reports for 2009 and 2010 are publically available and posted on NMFS' Office of Protected Resources website.

False killer whales are long-lived, slow to mature, apex predators, and therefore susceptible to bioaccumulation of persistent organic pollutants (POPs). Hawaiian waters are home to three distinct populations: pelagic; Northwestern Hawaiian Islands (NWHI) insular; and main Hawaiian Islands (MHI) insular. Following a precipitous decline over recent decades, the MHI population was listed as “endangered” under the Endangered Species Act in 2012. This study assesses the risk of POP exposure to these populations by examining pollutant concentrations and ratios from blubber samples (n = 56) related to life history characteristics and MHI social clusters.

Vessel Surveys and Visual Species: Verification Trials at the Jacksonville Shallow Water Training Range

Introgressive hybridization, in which genetic material from one species persists in the genome of another species following hybridization, can result in gene trees that do not match species trees and seriously mislead phylogenetic studies. This is particularly true in studies based solely on mitochondrial DNA, as introgression of maternally-inherited genomes is predicted to occur far more rapidly and persist longer than introgression of nuclear genomes. Introgressive hybridization has long been suspected as a source of taxonomic confusion in the Delphinidae. We detected mitochondrial introgressive hybridization in bottlenose dolphins (Tursiops truncatus) sampled around the southern islands of the Mariana Archipelago, where five out of 15 animals possess haplotypes characteristic of Fraser's dolphins (Lagenodelphis hosei). We used mitochondrial sequence data and genotypes from 14 nuclear microsatellite loci to further examine introgression of Fraser's dolphin DNA into the Mariana Islands population of bottlenose dolphins. We compared the Mariana Islands samples to bottlenose dolphin samples from elsewhere in the western Pacific and the Hawaiian Archipelago and to Fraser's dolphin samples from throughout the Pacific and Indian Oceans to investigate the extent and origin of hybrid ancestry in Mariana Islands bottlenose dolphins. We estimate that the Mariana Islands animals derive approximately 14% of their nuclear ancestry from Fraser's dolphins. The fact that every Mariana Islands sample showed evidence of nuclear introgression, combined with the fact that those exhibiting mitochondrial introgression all share the same Fraser's dolphin haplotype, suggests that there was a single hybridization event far enough in the past to allow Fraser's dolphin nuclear DNA to permeate the population. The Mariana Islands samples exhibited low genetic diversity compared to other bottlenose dolphin populations, suggesting that they represent a small, genetically isolated population.

Small, island-associated populations of cetaceans have evolved around numerous oceanic islands, likely due to habitat discontinuities between nearshore and offshore waters. However, little is known about the ecology and structure of cetacean populations around the Mariana Islands, a remote archipelago in the western Pacific Ocean. We present sighting, photo-identification, and genetic data collected during twelve years of surveys around these islands that reveal the existence of a small, island-associated population of bottlenose dolphins.

This work is in support of marine species monitoring activities by the United States (US) Navy in its training and testing range complexes in the Pacific Ocean. Specifically, the study seeks to help delineate humpback whale Distinct Population Segments found off the US West Coast, as well as to describe their feeding-season home range, migration to the breeding areas, diving behavior, habitat use, and ecological characteristics.

The purpose of this Cooperative Agreement between Naval Facilities Engineering Command Southwest (NAVFAC SW) and Oregon State University (OSU) is to support marine mammal studies in compliance with the Letters of Authorization and Biological Opinions issued by the National Marine Fisheries Service (NMFS) to the Navy for activities in all Pacific Ocean testing and training range complexes. With regard to humpback whales, in 2016 NMFS divided the global population into fourteen Distinct Population Segments (DPS) for purposes of listing under the Endangered Species Act (ESA). Four DPS were designated for the North Pacific based on the location of distinct breeding areas (Federal Register 2016a, b): “Western North Pacific”, “Hawaii”, “Mexico”, and “Central America”. The corresponding ESA status is “Endangered” for both the Western North Pacific (estimated at 1,066 animals; Wade 2017) and the Central America DPS (estimated at 783 animals; Wade 2017); “Threatened” for the Mexico DPS (estimated at 2,806 animals; Wade 2017); and “Not Listed” for the Hawaii DPS (estimated at 11,571 animals; Wade 2017) (Federal Register 2016a, b).

This report presents detailed results from the tagging, biopsy sampling, and photo-identification (photo-ID) efforts conducted in Hawaii in 2018, as well as results from previous OSU studies of humpback whales in Hawaii from 1995 to 2015. Whale use of Navy training and testing areas as well as their use of NMFS-identified Biologically Important Areas (BIAs) in Hawaii and Alaska is also examined.

This Preliminary Summary provides an overview of field survey methodologies as well as initial summaries of the data collected through 1 July 2019. Twenty-five tags were deployed and Argos satellite locations were received from 24 of them.

This report presents detailed results from the tagging, biopsy sampling, and photo-ID efforts conducted in Hawaii in 2018, as well as results from previous OSU studies of humpback whales in Hawaii from 1995 to 2015. Whale use of Navy training and testing areas as well as their use of NMFS-identified Biologically Important Areas (BIAs) in Hawaii and Alaska is also examined.

This report presents detailed results from the tagging, biopsy sampling, and photo-identification efforts conducted off southern and central California in summer and off Oregon in fall, as well as results from previous OSU studies of humpback whales in California, Oregon, Southeast Alaska, and the Aleutian Islands during 1997–2016.

As part of the U.S. Navy’s Atlantic Fleet Testing and Training program, we are monitoring the occurrence, distribution and density of cetaceans at three offshore sites along the U.S. Atlantic coast. We are using an identical suite of survey methods (line transect surveys, photoidentification, biopsy sampling and passive acoustic monitoring) at each 500nm2 reference site. Surveys began in Onslow Bay, NC in 2007, Jacksonville, FL in 2009 and Cape Hatteras, NC in 2011. To date we have conducted 48674km, 60687km, and 14213km of aerial surveys with 6883km, 3403km and 2443km of vessel surveys and 1201 days, 1264 days and 28 days of Highfrequency Acoustic Recording Package (HARP) recordings at Onslow, Jacksonville and Cape Hatteras, respectively

During January 2012, with funding provided by the Chief of Naval Operations (CNO) N45 Energy and Environmental Readiness Division, the Marine Mammal Monitoring on Navy Ranges ( M3R) program supported a marine mammal species verification and satellite tagging test on the Pacific Missile Range Facility (PMRF) prior to a Submarine Commander Course (SCC)

The U.S. Navy represented by the Pacific Fleet Environmental Readiness Division, Chief of Naval Operations Environmental Readiness and Energy, and Office of Naval Research fund significant marine mammal monitoring programs within Southern California. In an endeavor coordinated with the U.S. National Marine Fisheries Service to address specific questions, efforts are underway to deploy a variety of research tools as a collection of focused, collaborative studies. Monitoring methods include research elements designed to collect data in support of regionspecific monitoring and also contribute to the overall scientific body of marine mammal knowledge. Research elements include visual surveys from airplanes and vessels, passive acoustic monitoring from bottom-mounted systems, photoID, biopsies, and marine mammal tagging. The framework for monitoring and results from August 2008 to August 2012 will be presented. During this period over 76,400 nautical miles of ocean were visually surveyed representing 4,450 hours of effort. There were over 4,660 sightings of an estimated 338,875 marine mammals. Over 45,000 hours of passive acoustic recordings were obtained and are undergoing continued analysis. In addition, 64,693 digital photographs were taken of marine mammals, and 45 hours of digital video taken of various marine mammal behaviors. There were also 241 individual tissue biopsies, and 74 satellite tracking tags attached to various species. Continuing efforts in 2013 will employ many of the same techniques used previously. The Navy’s annual monitoring reports for 2009, 2010, and 2011 are publically available and posted on NMFS’ Office of Protected Resources website as well as a new public Navy monitoring website.

This report covers a number of activities carried out by the Cetacean Research Program (CRP) at the Pacific Islands Fisheries Science Center (PIFSC) in 2009 and 2010 supported with partial funding from U.S. Navy Pacific Fleet (PACFLT). The report is intended to provide preliminary data and results from 4 survey efforts between Hawaii and Guam in early 2010.

Since 2012, Oregon State University (OSU) has been conducting tagging and tracking studies on large whales in support of the U.S. Navy’s efforts to meet regulatory requirements for marine mammal monitoring under the Endangered Species Act and the Marine Mammal Protection Act. The primary goal of these studies has been to determine whale movement patterns, occurrence, and residence times within several Navy training and testing areas in the Pacific Ocean, as reported extensively in a series of previous Technical Reports

This report presents detailed results from the tagging, biopsy sampling, and photo-identification (photo-ID) efforts conducted in the Hawaiian Islands in 2019, as well as results from previous OSU studies of humpback whales in the Hawaiian Islands from 1995 to 2018. Whale use of Navy training and testing areas as well as their use of NMFS-identified Biologically Important Areas (BIAs) in the Hawaiian Islands and Alaska is also examined.

This report presents results from field efforts conducted off the coast of northern Washington (NWA) in summer and fall 2019, as well as results from previous OSU studies of humpback whales in southern and central California (SCCA), northern California and Oregon (NCA/OR), and NWA from 2004 to 2018.